Aptamer-based biosensors for the rapid visual detection of flu viruses.
نویسندگان
چکیده
RNA aptamers showing affinity and specificity for different strains of human influenza virus were assembled onto gold nanoparticles that subsequently formed a gold nanoshell (AuNS) around the viral envelope. These shells could be visualised by transmission electron microscopy (TEM). Changes in size and structure of the AuNS coated virus can be used to detect the viruses. We show that sedimentation with a low cost centrifuge and visual determination can detect 3 × 10(8) viral particles.
منابع مشابه
DNA Nanotubes Coupled with Magnetic Nanoparticles as a Platform for Colorimetric Biosensors
This study describes the fabrication techniques for two forms of magnetic DNA nanotubes (MDNTs) and their applications as platforms for developing colorimetric assays. The first form of MDNTs was DNTs filled-up with magnetic nanoparticles (MNPs) and the second one was DNTs arayed with MNPs on their extrior surfaces. Then the both forms of MDNTs were employed as platforms for attaching a specifi...
متن کاملAn Aptamer-based Biosensor for Troponin I Detection in Diagnosis of Myocardial Infarction
Background: Acute myocardial infarction (MI) accounts for one third of deaths. Cardiac troponin I (TnI) is a reliable biomarker of cardiac muscle tissue injury and is employed in the early diagnosis of MI.Objectives: In this study, a molecular method is introduced to early diagnosis of MI by rapid detection of TnI.Materials and Methods: The detection method was based on electrochemical aptasens...
متن کاملThe Use of Aptamer in Detection of Pathogenic Bacteria-
Detection, identification and measurement of microbial pathogens is critical for protecting public health. Although microbial culture-based tests and molecular techniques are currently the most commonly used, these techniques are time-consuming and require complex tools and experienced individuals. Consequently, it is costly to analyze these techniques. The emergence of the aptamer led to the e...
متن کاملMonitoring Intact Viruses Using Aptamers
Viral diagnosis and surveillance are necessary steps in containing the spread of viral diseases, and they help in the deployment of appropriate therapeutic interventions. In the past, the commonly employed viral detection methods were either cell-culture or molecule-level assays. Most of these assays are laborious and expensive, require special facilities, and provide a slow diagnosis. To circu...
متن کاملAptamer-Based Technologies in Foodborne Pathogen Detection
Aptamers are single stranded DNA or RNA ligands, which can be selected by a method called systematic evolution of ligands by exponential enrichment (SELEX); and they can specifically recognize and bind to their targets. These unique characteristics of aptamers offer great potentials in applications such as pathogen detection and biomolecular screening. Pathogen detection is the critical means i...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Chemical communications
دوره 50 98 شماره
صفحات -
تاریخ انتشار 2014